Recombinant:Article Title: Protocol to assess extravasation of fluorescent molecules in mice after ultrasound-mediated blood-brain barrier opening.
Article Snippet: GENT or RESOURCE SOURCE IDENTIFIER micals, peptides, and recombinant proteins fluorobutane (C4F10) F2 Chemicals Ltd. CAS: 355-25-9 tearoylphosphatidylcholine (DSPC) Avanti Polar Lipids 850365C, CAS: 816-94-4 0 PEG2000 PE Avanti Polar Lipids 880120, CAS: 474922-77-5 cerol Sigma-Aldrich G5516, CAS: 56-81-5 pylene glycol Sigma-Aldrich BP868, CAS: 57-55-6 ne Fisher Scientific Z1376, CAS: 7647-14-5 rose powder Holland Moran 400402500, CAS: 9002-18-0 becco’s phosphate-buffered saline Biological Industries Israel 02-023-1A urane United States Pharmacopeia 1349003, CAS: 26675-46-7 ns blue dye Sigma-Aldrich E2129, CAS: 314-13-6 orescein isothiocyanate–Dextran 2000 Sigma-Aldrich 52471, CAS: 60842-46-8 T cryo compound Leica Biosystems 14020108926 ethylbutane Merck-Sigma M32631 osited data mple microscopy data This paper https://zenodo.org/badge/latestdoi/637521961 erimental models: Organisms/strains ale C57BL/6 mice (10–11 week old, 16-21gr) Envigo C57BL/6JOlaHsd tware and algorithms TLAB MathWorks, Inc. https://www.mathworks.com/products/matlab.html id Editable Analysis of Vessel Elements tine (REAVER) Corliss, B.A. et al. https://github.com/uva-peirce-cottler-lab/public_REAVER (Continued on next page) STAR Protocols 5, 102770, March 15, 2024 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Image processing and data analysis software This paper https://zenodo.org/badge/latestdoi/637521961 Prism GraphPad https://www.graphpad.com/ Other AccuSizer FX-Nano, particle sizing systems Entegris A9000 AD 250 kHz, single element, focused ultrasound transducer Sonic Concepts H115 Arbitrary/Function generator Tektronix, Inc. AFG1012 Laboratory linear power amplifier E&I 2100L Transducer Power Output Sonic Concepts TPO-200 Needle hydrophone Precision Acoustics NH0500 Phased array ultrasound transducer Philips ATL P4-1 Vantage research ultrasound system Verasonics, Inc. Vantage 256 Low-flow electronic vaporizer Kent Scientific SomnoFlo Cryostat microtome Leica Biosystems CM1950 Automated fluorescence microscope Olympus Life Science BX63 20x microscope objective Olympus Life Science UPLFLN 20X ll OPEN ACCESS Protocol
Saline:Article Title: Protocol to assess extravasation of fluorescent molecules in mice after ultrasound-mediated blood-brain barrier opening.
Article Snippet: GENT or RESOURCE SOURCE IDENTIFIER micals, peptides, and recombinant proteins fluorobutane (C4F10) F2 Chemicals Ltd. CAS: 355-25-9 tearoylphosphatidylcholine (DSPC) Avanti Polar Lipids 850365C, CAS: 816-94-4 0 PEG2000 PE Avanti Polar Lipids 880120, CAS: 474922-77-5 cerol Sigma-Aldrich G5516, CAS: 56-81-5 pylene glycol Sigma-Aldrich BP868, CAS: 57-55-6 ne Fisher Scientific Z1376, CAS: 7647-14-5 rose powder Holland Moran 400402500, CAS: 9002-18-0 becco’s phosphate-buffered saline Biological Industries Israel 02-023-1A urane United States Pharmacopeia 1349003, CAS: 26675-46-7 ns blue dye Sigma-Aldrich E2129, CAS: 314-13-6 orescein isothiocyanate–Dextran 2000 Sigma-Aldrich 52471, CAS: 60842-46-8 T cryo compound Leica Biosystems 14020108926 ethylbutane Merck-Sigma M32631 osited data mple microscopy data This paper https://zenodo.org/badge/latestdoi/637521961 erimental models: Organisms/strains ale C57BL/6 mice (10–11 week old, 16-21gr) Envigo C57BL/6JOlaHsd tware and algorithms TLAB MathWorks, Inc. https://www.mathworks.com/products/matlab.html id Editable Analysis of Vessel Elements tine (REAVER) Corliss, B.A. et al. https://github.com/uva-peirce-cottler-lab/public_REAVER (Continued on next page) STAR Protocols 5, 102770, March 15, 2024 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Image processing and data analysis software This paper https://zenodo.org/badge/latestdoi/637521961 Prism GraphPad https://www.graphpad.com/ Other AccuSizer FX-Nano, particle sizing systems Entegris A9000 AD 250 kHz, single element, focused ultrasound transducer Sonic Concepts H115 Arbitrary/Function generator Tektronix, Inc. AFG1012 Laboratory linear power amplifier E&I 2100L Transducer Power Output Sonic Concepts TPO-200 Needle hydrophone Precision Acoustics NH0500 Phased array ultrasound transducer Philips ATL P4-1 Vantage research ultrasound system Verasonics, Inc. Vantage 256 Low-flow electronic vaporizer Kent Scientific SomnoFlo Cryostat microtome Leica Biosystems CM1950 Automated fluorescence microscope Olympus Life Science BX63 20x microscope objective Olympus Life Science UPLFLN 20X ll OPEN ACCESS Protocol
Microscopy:Article Title: Protocol to assess extravasation of fluorescent molecules in mice after ultrasound-mediated blood-brain barrier opening.
Article Snippet: GENT or RESOURCE SOURCE IDENTIFIER micals, peptides, and recombinant proteins fluorobutane (C4F10) F2 Chemicals Ltd. CAS: 355-25-9 tearoylphosphatidylcholine (DSPC) Avanti Polar Lipids 850365C, CAS: 816-94-4 0 PEG2000 PE Avanti Polar Lipids 880120, CAS: 474922-77-5 cerol Sigma-Aldrich G5516, CAS: 56-81-5 pylene glycol Sigma-Aldrich BP868, CAS: 57-55-6 ne Fisher Scientific Z1376, CAS: 7647-14-5 rose powder Holland Moran 400402500, CAS: 9002-18-0 becco’s phosphate-buffered saline Biological Industries Israel 02-023-1A urane United States Pharmacopeia 1349003, CAS: 26675-46-7 ns blue dye Sigma-Aldrich E2129, CAS: 314-13-6 orescein isothiocyanate–Dextran 2000 Sigma-Aldrich 52471, CAS: 60842-46-8 T cryo compound Leica Biosystems 14020108926 ethylbutane Merck-Sigma M32631 osited data mple microscopy data This paper https://zenodo.org/badge/latestdoi/637521961 erimental models: Organisms/strains ale C57BL/6 mice (10–11 week old, 16-21gr) Envigo C57BL/6JOlaHsd tware and algorithms TLAB MathWorks, Inc. https://www.mathworks.com/products/matlab.html id Editable Analysis of Vessel Elements tine (REAVER) Corliss, B.A. et al. https://github.com/uva-peirce-cottler-lab/public_REAVER (Continued on next page) STAR Protocols 5, 102770, March 15, 2024 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Image processing and data analysis software This paper https://zenodo.org/badge/latestdoi/637521961 Prism GraphPad https://www.graphpad.com/ Other AccuSizer FX-Nano, particle sizing systems Entegris A9000 AD 250 kHz, single element, focused ultrasound transducer Sonic Concepts H115 Arbitrary/Function generator Tektronix, Inc. AFG1012 Laboratory linear power amplifier E&I 2100L Transducer Power Output Sonic Concepts TPO-200 Needle hydrophone Precision Acoustics NH0500 Phased array ultrasound transducer Philips ATL P4-1 Vantage research ultrasound system Verasonics, Inc. Vantage 256 Low-flow electronic vaporizer Kent Scientific SomnoFlo Cryostat microtome Leica Biosystems CM1950 Automated fluorescence microscope Olympus Life Science BX63 20x microscope objective Olympus Life Science UPLFLN 20X ll OPEN ACCESS Protocol
Software:Article Title: Protocol to assess extravasation of fluorescent molecules in mice after ultrasound-mediated blood-brain barrier opening.
Article Snippet: GENT or RESOURCE SOURCE IDENTIFIER micals, peptides, and recombinant proteins fluorobutane (C4F10) F2 Chemicals Ltd. CAS: 355-25-9 tearoylphosphatidylcholine (DSPC) Avanti Polar Lipids 850365C, CAS: 816-94-4 0 PEG2000 PE Avanti Polar Lipids 880120, CAS: 474922-77-5 cerol Sigma-Aldrich G5516, CAS: 56-81-5 pylene glycol Sigma-Aldrich BP868, CAS: 57-55-6 ne Fisher Scientific Z1376, CAS: 7647-14-5 rose powder Holland Moran 400402500, CAS: 9002-18-0 becco’s phosphate-buffered saline Biological Industries Israel 02-023-1A urane United States Pharmacopeia 1349003, CAS: 26675-46-7 ns blue dye Sigma-Aldrich E2129, CAS: 314-13-6 orescein isothiocyanate–Dextran 2000 Sigma-Aldrich 52471, CAS: 60842-46-8 T cryo compound Leica Biosystems 14020108926 ethylbutane Merck-Sigma M32631 osited data mple microscopy data This paper https://zenodo.org/badge/latestdoi/637521961 erimental models: Organisms/strains ale C57BL/6 mice (10–11 week old, 16-21gr) Envigo C57BL/6JOlaHsd tware and algorithms TLAB MathWorks, Inc. https://www.mathworks.com/products/matlab.html id Editable Analysis of Vessel Elements tine (REAVER) Corliss, B.A. et al. https://github.com/uva-peirce-cottler-lab/public_REAVER (Continued on next page) STAR Protocols 5, 102770, March 15, 2024 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Image processing and data analysis software This paper https://zenodo.org/badge/latestdoi/637521961 Prism GraphPad https://www.graphpad.com/ Other AccuSizer FX-Nano, particle sizing systems Entegris A9000 AD 250 kHz, single element, focused ultrasound transducer Sonic Concepts H115 Arbitrary/Function generator Tektronix, Inc. AFG1012 Laboratory linear power amplifier E&I 2100L Transducer Power Output Sonic Concepts TPO-200 Needle hydrophone Precision Acoustics NH0500 Phased array ultrasound transducer Philips ATL P4-1 Vantage research ultrasound system Verasonics, Inc. Vantage 256 Low-flow electronic vaporizer Kent Scientific SomnoFlo Cryostat microtome Leica Biosystems CM1950 Automated fluorescence microscope Olympus Life Science BX63 20x microscope objective Olympus Life Science UPLFLN 20X ll OPEN ACCESS Protocol
Fluorescence:Article Title: Protocol to assess extravasation of fluorescent molecules in mice after ultrasound-mediated blood-brain barrier opening.
Article Snippet: GENT or RESOURCE SOURCE IDENTIFIER micals, peptides, and recombinant proteins fluorobutane (C4F10) F2 Chemicals Ltd. CAS: 355-25-9 tearoylphosphatidylcholine (DSPC) Avanti Polar Lipids 850365C, CAS: 816-94-4 0 PEG2000 PE Avanti Polar Lipids 880120, CAS: 474922-77-5 cerol Sigma-Aldrich G5516, CAS: 56-81-5 pylene glycol Sigma-Aldrich BP868, CAS: 57-55-6 ne Fisher Scientific Z1376, CAS: 7647-14-5 rose powder Holland Moran 400402500, CAS: 9002-18-0 becco’s phosphate-buffered saline Biological Industries Israel 02-023-1A urane United States Pharmacopeia 1349003, CAS: 26675-46-7 ns blue dye Sigma-Aldrich E2129, CAS: 314-13-6 orescein isothiocyanate–Dextran 2000 Sigma-Aldrich 52471, CAS: 60842-46-8 T cryo compound Leica Biosystems 14020108926 ethylbutane Merck-Sigma M32631 osited data mple microscopy data This paper https://zenodo.org/badge/latestdoi/637521961 erimental models: Organisms/strains ale C57BL/6 mice (10–11 week old, 16-21gr) Envigo C57BL/6JOlaHsd tware and algorithms TLAB MathWorks, Inc. https://www.mathworks.com/products/matlab.html id Editable Analysis of Vessel Elements tine (REAVER) Corliss, B.A. et al. https://github.com/uva-peirce-cottler-lab/public_REAVER (Continued on next page) STAR Protocols 5, 102770, March 15, 2024 3 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Image processing and data analysis software This paper https://zenodo.org/badge/latestdoi/637521961 Prism GraphPad https://www.graphpad.com/ Other AccuSizer FX-Nano, particle sizing systems Entegris A9000 AD 250 kHz, single element, focused ultrasound transducer Sonic Concepts H115 Arbitrary/Function generator Tektronix, Inc. AFG1012 Laboratory linear power amplifier E&I 2100L Transducer Power Output Sonic Concepts TPO-200 Needle hydrophone Precision Acoustics NH0500 Phased array ultrasound transducer Philips ATL P4-1 Vantage research ultrasound system Verasonics, Inc. Vantage 256 Low-flow electronic vaporizer Kent Scientific SomnoFlo Cryostat microtome Leica Biosystems CM1950 Automated fluorescence microscope Olympus Life Science BX63 20x microscope objective Olympus Life Science UPLFLN 20X ll OPEN ACCESS Protocol
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